RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free NB200-598 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemRNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price Pricing Info Supplier Page View Supplier Product Page
Catalog NumberNB200-598
Immunogen10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProt# P24928]
Concentration1 mg/ml
HostMouse
IsotypeIgG1
FormatPBS
Clone4H8
ClonalityMonoclonal
Quantity0.1 ml (also 0.025 ml)
ReactivityHuman, Mouse, Yeast
TargetRNA Polymerase II/POLR2A
ApplicationsWestern Blot, Flow Cytometry, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Flow (Intracellular), Chromatin Immunoprecipitation (ChIP), CyTOF-ready
Conjugate/TagUnconjugated
Brand/TrademarkNovus Biologicals, a Bio-Techne Brand
PurityProtein G purified
Molecular Weight217 kDa
Research AreaChromatin Research, Epigenetics, Transcription Factors and Regulators
Antibody TypePrimary Antibodies
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles
NCBI Full Gene NameRNA polymerase II subunit A
NCBI Gene AliasesRPO2, RPB1, RPOL2, POLRA, hsRPB1, hRPB220, POLR2, RpIILS, NEDHIB, RPBh1

Specifications/Features

Available conjugates: Unconjugated

Specificity: Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein

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Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: http://www.novusbio.com

(1) Transgenic mice lacking FGF15/19-SHP phosphorylation display altered bile acids and gut bacteria, promoting nonalcoholic fatty liver diseaseThe Journal of biological chemistryAugust 1, 2023Kim YC, Qi M, Dong X, Seok S, Sun H, Kemper B, Fu T, Kemper JK.and re-precipitated by antibody against SHP (Abcam, ab96605) or RNA polymerase II (Novus biologicals, NB200–598). DNA was isolated and quantified by qPCR using a primer set for detecting SHP and RNA pol II occupancies

(2) TEAD1 regulates cell proliferation through a pocket-independent transcription repression mechanismNucleic acids researchDecember 9, 2022Li F, Negi V, Yang P, Lee J, Ma K, Moulik M, Yechoor VK.

(3) The Taspase1/Myosin1f-axis regulates filopodia dynamicsiScienceJune 17, 2022Hensel A, Stahl P, Moews L, König L, Patwardhan R, Höing A, Schulze N, Nalbant P, Stauber RH, Knauer SK.α,Sigma,Cat#: T6074;RRID: AB_477582 Mouse monoclonal anti-RNA Polymerase II/POLR2A,Novus Biologicals,Cat#: NB200-598;RRID: AB_2167465 Mouse monoclonal anti-Histone H3,Abcam,Cat#: ab195277;RRID: AB_2885180 "Sheep anti-mouse

(4) EZH2 engages TGFβ signaling to promote breast cancer bone metastasis via integrin β1-FAK activationNature communicationsMay 10, 2022Zhang L, Qu J, Qi Y, Duan Y, Huang YW, Zhou Z, Li P, Yao J, Huang B, Zhang S, et al.IgG (sc-2025, sc-2027) were purchased from Santa Cruz Biotechnology. Antibodies against RNA Pol II (NB200-598) were purchased from Novus Biologicals. The antibody against phospho-tyrosine (#610000) was purchased

(5) Sen1 Is Recruited to Replication Forks via Ctf4 and Mrc1 and Promotes Genome StabilityCell reportsFebruary 18, 2020Appanah R, Lones EC, Aiello U, Libri D, De Piccoli G.

(6) Cyclic Multiplexed-Immunofluorescence (cmIF), a Highly Multiplexed Method for Single-Cell AnalysisMethods in molecular biology (Clifton, N.J.)January 1, 2020Eng J, Thibault G, Luoh SW, Gray JW, Chang YH, Chin K.

(7) Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopeseLifeJuly 11, 2018Lin JR, Izar B, Wang S, Yapp C, Mei S, Shah PM, Santagata S, Sorger PK.

(8) PRDX2 and PRDX4 are negative regulators of hypoxia-inducible factors under conditions of prolonged hypoxiaOncotargetFebruary 9, 2016Luo W, Chen I, Chen Y, Alkam D, Wang Y, Semenza GL.(NB100-124, Novus Biologicals), HIF-2α (NB100-122, Novus Biologicals), and RNA polymerase II (pSer5) (NB200-598, Novus Biologicals). PCR primer sequences are shown in Table 1. Statistical analysis Data are expressed

(9) Peripheral subnuclear positioning suppresses Tcrb recombination and segregates Tcrb alleles from RAG2Proceedings of the National Academy of Sciences of the United States of AmericaNovember 26, 2013Chan EA, Teng G, Corbett E, Choudhury KR, Bassing CH, Schatz DG, Krangel MS.Jackson ImmunoResearch Laboratories). PolII was visualized using a monoclonal mouse anti-PolII antibody (NB200-598; Novus Biologicals) and FITC-conjugated donkey anti-mouse IgG (715-545-151; Jackson ImmunoResearch Laboratories).

(10) Spt6 prevents transcription-coupled loss of posttranslationally modified histone H3Scientific reportsJanuary 1, 2013Kato H, Okazaki K, Iida T, Nakayama J, Murakami Y, Urano T.

(11) Plasma membrane associated transcription of cytoplasmic DNAProceedings of the National Academy of Sciences of the United States of AmericaJuly 3, 2012Cheng J, Torkamani A, Peng Y, Jones TM, Lerner RA.

(12) Proteasome regulation of dynamic transcription factor occupancy on the GnRH-stimulated luteinizing hormone beta-subunit promoterMolecular endocrinology (Baltimore, Md.)February 1, 2009Walsh HE, Shupnik MA.

(13) DNA damage-induced Def1-RNA polymerase II interaction and Def1 requirement for polymerase ubiquitylation in vitroThe Journal of biological chemistryJuly 16, 2004Reid J, Svejstrup JQ.

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RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free

RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free

RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free

RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free

RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free

RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free from Novus Biologicals, a Bio-Techne Brand

Specifications/Features

Novus Biologicals, a Bio-Techne Brand

Citations (13)

Reviews

RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free from Novus Biologicals, a Bio-Techne Brand

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